Construction of a dose – response curve by induction of premature chromosome condensation for biological dosimetry

نویسندگان

  • H. Mozdarani
  • S. Mohammadifrad
چکیده مقاله:

Premature Chromosome Condensation (PCC) appears to have a possible utility for biological dosimetry purposes. The PCC technique may be adapted for cases of suspicion of overexposure where sampling is performed at least one day after an accident. For this purpose, human blood samples were exposed in vitro to 60Co up to 10 Gy and the PCC technique was performed immediately after irradiation. Analysis of excess PCC fragments distribution showed an over dispersion and the dose- effect relationship was best characterized by linear regression. Iran. J. Radiat. Res., 2009 6 (4): 213-218

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

construction of a dose – response curve by induction of premature chromosome condensation for biological dosimetry

premature chromosome condensation (pcc) appears to have a possible utility for biological dosimetry purposes. the pcc technique may be adapted for cases of suspicion of overexposure where sampling is performed at least one day after an accident. for this purpose, human blood samples were exposed in vitro to 60co up to 10 gy and the pcc technique was performed immediately after irradiation. anal...

متن کامل

Biological Dose Assessment by Chemical Induction of Premature Chromosome Condensation

Dose assessment of radiation victims is a key element in medical management of radiation accidents. At high radiation doses (> 6 Gy), dicentric assay lose its efficiency and premature chromosome condensation (PCC) assay was proposed to overcome the restrictions of dicentric assay. It was shown that PCC ring is more suitable and simpler than dicentric for biodosimetry in high radiation doses. In...

متن کامل

Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry

Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. Materials and Methods: Peripheral blood was obtained from a healthy donor and expo...

متن کامل

Construction of Calibration Curve for Premature Chromosome Condensation Assay for Dose Assessment

Cytogenetic dosimetry plays an important role in the triage and medical management of affected people in radiological incidents/accidents. Cytogenetic biodosimetry uses different methods to estimate the absorbed dose in the exposed individuals, and each approach has its advantages and disadvantages. Premature chromosome condensation (PCC) assay presents several advantages that hopefully fulfill...

متن کامل

induction of premature chromosome condensation (pcc) by calyculin a for biodosimetry

background: premature chromosome condensation (pcc) is a method for interphase chromosome analysis in biodosimetry. this study was performed to verify the usefulness of pcc induced by calyculin a in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. materials and methods: peripheral blood was obtained from a healthy donor and expo...

متن کامل

Determination of dose-response relationship in cultured human lymphocytes for biological dosimetry

Background: Lymphocyte-dicentric assay is the most generally accepted method for biological dosimetry of overexposed individuals. In this study, the frequency of unstable chromosome aberration in blood lymphocytes was used to estimate radiation dose received by individuals. Evaluation of dose using a calibration curve produced elsewhere may have a significant uncertainty therefore, experiments ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 6  شماره None

صفحات  213- 218

تاریخ انتشار 2009-03

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023